water sampling from closed plastic liner with water
saturated marine sediment by drilling 3.8mm holes and
gently inserting rhizon pore water samplers.
with disposable 20 mL syringes. Syringe holds
vacuum by keeping plunger in place with wooden spacer.
Rhizons are 5cm flat tip 19.21.23
F Rhizon CSS rhizons
with female adapter (blue).
you stick rhizons into sediment, be sure to hold the
tube together with the supporting fiberglass stick
firmly between your fingers and thumb ! Do not push the
rhizon from the connecting tube between porous part
(white) and tubing (transparent) before the rhizon is
fully in the sediment.
sediment is stiff or sandy, use a 2.5mm stainless steel
or plastic stick (knitting needle works) to prepare a
of connecting tube is 0.15" or 3.8 mm so it seals
perfectly in a 3.8mm hole
the application above we use a special flat tip version
of the Rhizones( Rhizon
CSS - Core Solution Sampler), where tip diameter =
porous tubing diamter, so the contact between sediment
and sampler is very smooth in soft sediments. If
sediment is stiff a hole has to be prepared before
insertion in order to protect the microporous tube from
breaking (i.e. knitting needle of appropiate diameter).
Order numbers from Rhizosphere Research Products -
19.21.01 F Rhizon SMS 10 cm stainless steel support.
(pack of 10)
19.21.05 F Rhizon
SMS 5 cm stainless steel support (pack of 10)
19.21.21 F Rhizon MOM 10 cm metal-free support. (pack of
19.21.22 F Rhizon MOM 5 cm metal-free support. (pack of
The "Bremen flat tip version" or "Koelling version" now
has a separate order number:
19.21.23 F Rhizon
CSS 5 cm metal-free support. (pack of 10)
all Rhizons add "F" to the order number if you want a
female luer connector (for directly connecting syringe)
instead of the standard male connector
(for connecting needle and vacutube)
Rhizons are already widely used in soil science for
sampling from non-water-saturated environments. This is
possible since - once wettened - the microporous tube is
permeable only for liquids - thus keeping attached
vacuum in a dry environment.
There is also a porous ceramic (pure Al2O3) version of
moisture samplers called Rhizocera (pore width of 0.7
µm, diameter 3mm, length of 50 mm, flow 1ml/min/bar,
female luer adapter). (Rhizosphere Research Products
order No. 19.21.61)
and M.van Nordwijk (1992): Rhizon Soil
Solution Samplers as artificial roots. In: L. Kutschera
et. al (Eds.): Root Ecology and its practical
Application.- Proc 3rd ISSR Symp., 2.-6. Sep 1991, Wien
Austria; pp 793-795.
Seeberg-Elverfeldt, J., and M. Schlüter.
patent pended. Probennahmegerät zur simultanen Sammlung
von Porenwasser aus benachbarten aquatischen
Sedimentbereichen und Anwendung davon - Sampling device
for simultaneous collection of pore water from related
aquatic sediment areas and applications about it.
Reference number: 10 2004 053 111.0-24. Germany
J, Schlüter, M, Feseker, T & Kölling, M (2005):
Rhizon sampling of pore waters near the sediment/water
interface of aquatic systems, Limnology and
oceanography: Methods, 3, 361-371.
M., Seeberg-Elverfeldt, J., Schlüter, M.(2005).
Rhizon - an excellent pore water sampler for low
maintenance collection and filtration of small volume
samples, EGU General Assembly, 25-29 Apr., Vienna,
Seeberg-Elverfeldt, J., Schlüter, M., Kölling,
M., Feseker, T.(2005). New Rhizon in situ
sampler for pore water studies in aquatic sediments:
For example nutrient input from submarine groundwater
discharge in costal areas., EGU General Assembly,
25-29 Apr., Vienna, Austria. Abstract
Seeberg-Elverfeldt, J., Kölling, M., Schlüter,
M., Feseker, T.(2005). Rhizon in situ
sampler (RISS) for pore water sampling from aquatic
sediments., In-situ Methods and Investigations in
Environmental Science, 230th Meeting of the American
Chemical Society, Division of Geochemistry, 28 Aug. -
1 Sept., Washington, DC, USA
G.R., Koelling, M., Smith, D.C., Schnieders, L. and
the IODP Expedition 302 Scientists (2007):
Rhizon Sampling of Pore Waters on Scientific Drilling
Expeditions: An Example from the IODP Expedition 302,
Arctic Coring Expedition (ACEX).- Scientific Drilling
4: 22-25, doi:10.2204/iodp.sd.4.08.2007