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                     For our Teflon tape Flow injection NH4
                        analyzer after Hall
                        & Aller we use either an Amber Science 1056
                      conductivity meter with a 529 flow-through cell or an
                      Amber Science 3082 conductivity meter with a 829
                      flow-through cell. 
                       
                      Unfortunately, these wonderful little instruments have
                      been discontinued.  
                      As it seems to be hard to find information, we post
                      everything we know about them here: 
                     
                    Amber Science 1056 conductivity meter manual (pdf) 
                      Amber Science 3082 conductivity meter manual (pdf) 
                       
                      Amber Science 529 and 829 flow-through-cell drawing (pdf) 
                      Amber Science  829 flow-through-cell plug wiring (pdf) 
                         
                       
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                     Opening the conductivity cell housing
                     Caution: Do not turn the four large hex
                      screws on the side (5/32" / 4mm Allen). They hold the
                      measuring cell together. If you untighten those, the cell
                      starts leaking and ultimately falls apart! You can fix
                      that, but you will have to take off the case first (which
                      was what you originally wanted to do).  
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                    829 cell from outside: Four small Allen screws
                      (1/16" 1.6mm) fix the cell in the cylindrical case. You
                      have to peel off stickers to see them. The Phillips screws
                      are used to fix the whole cell in a box. Take them off (!)
                      before sliding cell out.  
                      Mark the position of the cell in the cylinder case. The
                      screws that fix the 829 conductivity cell in the
                      cylindrical housing are four small (1/16" = 1.6mm) grub
                      screws that can be accessed through holes in the cylinder.
                      These screw holes are covered by stickers that you have to
                      peel off.  
                     
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                     Opened conductivity cell:  Left:
                      829 cell. You can see the grub screws turned into the
                      aluminum plate (1/16 "= 1.6mm Allen). To separate the cell
                      from the cylinder, turn them clockwise! into the body!
                      Before sliding out the cell, unscrew the Phillips screw(s)
                      (on the outside of the cylinder), if there are any,
                      otherwise it will be hard to slide the cell out of the
                      cylinder.  
                      The white disk is already disassembled  
                      Right: detailed view of the face of the capillary
                      which is glued into a black plastic disc.  
                     
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                    Conductivity cell inside:
                     The flow-through conductivity cell from Amber Scientific
                      basically consists of two 1/16" stainless steel
                      capillaries, each with one (529 cell) or two ( 829 cell)
                      connecting wires and a temperature sensor that is glued
                      into the capillary holder.  
                      A white plastic (Delrin?) disk is clamped between the two
                      capillaries and represents the measuring cell length.  
                      The plastic disk is 8mm (5/16 ") thick, the bore is 1 mm
                      (0.04"),so the opening has a volume of 6.6 µL. The
                      total length of both capillaries and the bore is 5.5" or
                      140 mm so the total volume of the amber cell with
                      connection capillaries is about 116 µL.  
                      The connection capillaries are straight standard HPLC
                      capillaries with OD 1/16 "(= 1.6mm), ID 0.04" (= 1mm) and
                      they are 66mm (2.6") long.  
                      The inner sides of the capillaries on both sides of the
                      white disk represent the electrode surfaces. According to
                      the manufacturer the cell constant is 100, i.e. only the
                      last 0.25 mm (1/10") on each side effectively act as
                      electrode surface.  
                      This corresponds to an area of 0.8 mm2 (a
                      factor of 0.008 to the standard area of 100 mm2 with cell
                      constant 1). The cell length of 8 mm corresponds to 0.8
                      times the nominal distance of the standard cell (10 mm).
                      At 0.25 mm of the inner capillary wall length effectively
                      used as an electrode the cell constant is 0.8 / 0.008 =
                      100.  
                    The type 529 cell has a 6kOhm @ 25°C NTC as
                      temperature sensor and a 5-pin 180° DIN plug. 
                      The type 829 cell has a double 6kOhm / 30kOhm @ 25 °C
                      NTC as temperature sensor and an 8-pin 270 ° DIN plug
                      (with central pin). 
                      This means that the 829 cell may be connected to an old
                      Amber 1056 measuring device via an adapter (wiring see
                      below - you cannot buy this adapter). The other way (type
                      529 cell connected to 3082 meter) it doesn't really work,
                      unless you simulate the double wires by connecting the two
                      pins in the 8-pin connector. The second NTC could be
                      soldered directly into the connector - that is not quite
                      the temp on the capillary, but it might work for our
                      purposes. Otherwise you would have to convert the 529 into
                      an 829 which means changing the plug and the cable,
                      connecting it with 2 cables per capillary and replacing
                      the 6kOhm NTC with a 6k/30k double NTC or adding a 30kOhm
                      NTC.  
                      The conductivity wire connections are made using soldered
                      connections that are glued into the Delrin capillary
                      holder with the capillaries. Two soldering tails go to the
                      capillaries, but the pins are only intended as soldering
                      points for the thermistor cables which are not (!)
                      connected to the soldering tails.  
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                     Opened cells:  white plastic disc removed.
                      Left: 829 cell.  Right: 529 cell from
                      2005 (photo credit Sung-Uk An)  
                     
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                    Re-Assembly of the 529 or 829 flow-through conductivity
                      cell
                     Carefully center the white plastic disc between the
                      capillaries and tighten all four large Allen screws (5/32"
                      4mm) just enough that the disc remains a bit loose. Since
                      at least three of the screws have Loctite, you have little
                      feeling for how tight the screws actually are!  
                      One after the other - or rather crosswise - tighten all
                      four screws just enough to hold the white disk, and then
                      turn them all back a bit so that the white disk is
                      loose again.  
                      Use a slightly less than 1mm (0.04") wire to align the
                      white disk with the capillaries. A thick (= 0.9mm) nylon
                      wire (fishing line?) or a 0.036" guitar string (that
                      should be the fifth or A string for an electric guitar) or
                      maybe a 1/32" (=0.8mm) PEEK capillary will work fine as
                      well. You might also use the L-shaped cleaning wire that
                      comes with the cell. 
                      Simply stick the wire gently through the capillary, the
                      disk and the second capillary. This only works, if the
                      disk is loose enough. Any bit of misalignment will stop
                      the wire. Then tighten all four screws a little crosswise
                      again (approx. 1/4 turn).  
                    Now everything should be straight and tight. (don't
                      fasten screws too tight - it has to be very straight and
                      aligned, not very tight !!!).  
                      If you just turn the big Allen screws by feeling, it will
                      almost certainly be bending the connection a bit and the
                      cell will be leaking. 
                       
                       Check for leaks: (preferably before refitting the
                        cylinder case!!)   
                      Place the cell on kitchen paper or paper towels so you see
                      it leaking. Fill two disposable syringes half way with
                      water and attach one to each end of the two capillaries
                      with a short tube (should seal well). E.g. the tube end
                      with a female luer-lock
                        connector from a used rhizon
                      works very well).  
                      If all is fine, you should be able to push the water from
                      one syringe into the other and, if necessary, apply
                      pressure to both syringes. if not, it leaks immediately
                      (hence the cell on kitchen paper.).  
                      If it leaks: Unfasten the screws and carefully try
                      to reassemble so the white disk sits perfectly flat and
                      centered between the black pieces.  
                      When everything is "leakproof", insert the cell back into
                      the cylinder and carefully unscrew (= turn them
                      counterclockwise!) the grub screws crosswise (!) (1/16
                      "Allen key) so that the cell unit is centered the
                      cylinder. The grub screws press against the cylinder from
                      the inside so to fix the cell they have to be turned
                      counterclockwise.  
                    As you see, there is not much that could break in those
                      cells. As long as the capillaries are not blocked and the
                      inner wall of the capillary does not have stains, the
                      cells should work properly. Cleaning may be done with the
                      L-shaped wire provided with the cell or the same wire tha
                      you use for centering the white disk. 
                      
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                     Amber Science 3082 Conductivity Meter: Serial port
                      (RS232)
                     General remark: Only the 3082 model has a digital
                      output. The serial port only delivers exactly what you see
                      on the display. For the setup as a detector in a
                      flow-injection NH4 or CO2 detector
                      it is more precise to use the analogue signal because you
                      have a high background conductivity that prevents the
                      display from showing enough decimals. 
                     
                    Digital recording of data:
                     Required cable: serial extension (male - female SubD
                      9-pin) 
                      When you send CR or LF CR to the meter
                      through the serial connection (or automatically if in
                      auto-transmit mode) the device outputs data with the
                      following syntax: (CR = carriage return - hex0D, LF
                      = line feed - hex 0A) 
                     
                    computer: CRLF 
                       
                    Answer from meter:  
                      Data point xxxLF data point number. 
                      Conductivity xx.xx uSLF  5
                        digits, could also be x.xxx or xxx.x. µS or mS 
                      Temperature xxx.x Deg CLFCR  2
                        leading spaces  
                     
                     Auto transmit function  
                     When On: Values are automatically transmitted if
                      something changes (at least 2 digits) or if you push the
                      right dial. Settings are transmitted when the left switch
                      is in "self test" and the right dial is pushed. Parameters
                      (cell const etc.) cannot be changed 
                      When Off: Parameters may be changed. Output of
                      data when CR (= hex 0D) is sent from computer
                      program to RS232 
                     
                      
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                     Left: startup output from 3082 serial port. Right:
                      data point output after each CR sent to the
                      serial port 
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                     The Amber scientific software for the 3082 
                    There is a DOS / XP software that comes with the device
                      that can write data to files 
                      Very basic, works on DOS and Windows XP  
                      Alt-R:  write to file (new file or append if file
                      exists)  
                      Alt-F:  format .. as a standard 3 lines are output  
                      to output all info in one line (easier to read) press
                      Alt-F 1 (not the function key F1!)  
                      Alt-Q: End file (not quit program like it is standard now)
                       
                      Esc:   Quit = exit program completely. The file
                      is also closed.  
                      We did not check, but the program should also write
                      directly to a serial printer. 
                    But as mentioned above: Only the digits that can be seen
                      on the display are transmitted digitally. Thus the
                      analogue output is actually much more accurate! The major
                      problem that we have when we use it as a detector in a NH4
                      flow-injection system is that the background
                      conductivities are high. We use old Knauer strip chart
                      recorders that can compensate for quite a bit (but not
                      all) of zero offset.   
                    A better way would be to directly compensate for the
                      background conductivity by modifying the setup to become a
                      Wheaton bridge (parallel to measuring cell R1, a second
                      cell R2 with pure eluent). With a fixed resistor R3 and an
                      adjustable resistor R4 you should be able to adjust R1 /
                      R2 = R3 / R4. So if there is pure eluent in both
                      conductivity cells, the current should be 0 if the
                      resistances R3 and R4 are identical  
                       
                      There are precise HPLC conductivity meters that do exactly
                      this - but they are more expensive than the Amber meters
                      were. 
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                     Wiring for adapters:
                     Adapter : 829 cell (270° DIN with central pin) ->
                      1056 meter (5 pin 180° DIN) 
                     1 and 6 -> 1 electrode 1  
                       3 and 7 -> 3 electrode 2  
                             8 -> 2 ground  
                             5 -> 5 6k NTC  
                             2 -> 4 NTC common  
                             4 not connected (30k NTC)  
                    
                     Adapter : 529 cell - > 3082 meter (8 pin 270°
                      DIN) this is not precise, but might be working  
                     
                              1 -> 1
                      electrode 1 cable 1  
                                1
                      -> 6 electrode 1 cable 2 
                                2
                      -> 8 ground  
                                3
                      -> 3 electrode 2 cable 1 
                                3
                      -> 7 electrode 2 cable 2 
                                4
                      -> 2 NTC ground  
                                5
                      -> 5 6k NTC  
                      NTC 30k gnd -> 2  
                          NTC 30k -> 4
                     Adapter 829 -> WTW LF 191
                      (7pin 6x60° DIN w central pin)  
                          4 -> 1 NTC 30k
                      (829=red)  
                            5 -> 2 NTC 6K
                      (829=green)  
                            2 -> 3 NTC common
                      (829=yellow)  
                            1 -> 4 electrode 1 cable
                      1 (829=brown)  
                            6 -> 5 electrode 1 cable
                      2 (829=blue)  
                            3 -> 6 electrode 2 cable
                      1 (829=orange)  
                            7 -> 7 electrode 2 cable
                      2 (829=violet)  
                            8 -> shield (829=blank /
                      Adapter: black) 
                      
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                      time stamp 03Feb21  
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